FULL Acronis Backup Advanced 12.7.50089 Key ((FREE))

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Published: June 10, 2022 (2 weeks ago)
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Characterization of a bovine dendritic cell subset highly expressing Langerin that is a novel mucosal dendritic cell subset.
The differential expression of cell surface adhesion and costimulatory molecules is one mechanism by which mucosal dendritic cells (DC) contribute to the induction and maintenance of a state of tolerance to commensal bacteria. Langerhans cells (LC) are a unique subset of mucosal DC specialized to capture and present antigens to naive T cells. In addition to this well-characterized role, LC share many properties with tissue DC in that both populations express CD86, CD40, and MHC class II at high levels. In contrast, langerin(+) DC (Langerin(+) DC) express none of these molecules. Here we describe the morphological, phenotypic, and functional characteristics of this novel bovine LC/Langerin(+) DC subset. Langerin(+) DC were found to constitute a novel DC subset which is highly express CD86 and MHC class II and to traffic to draining lymph nodes, yet to have a functional capacity to induce antigen-specific T cell proliferation. These bovine Langerin(+) DC cells could be identified in peripheral tissues after intradermal inoculation of peptide antigen. While both CD1(+) and CD14(+) cells expressed both langerin and MHC class II on their surface, only a subset of CD1(+) and CD14(+) cells expressed high levels of langerin. The morphology and the distribution pattern of langerin(+) cells among skin-draining lymph nodes was similar to that of conventional LC. Our results demonstrate that Langerin(+) DC constitute a unique population of mucosal DC with a specialized role in T cell activation.Q:

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